Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2006
[Quantification for biofilm formation of pseudomonas aeruginosa isolates and their genotypic analysis].
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Cheng Xi, Yang Wei-qing, Shi Lei, Yin Xiao-lin, Xie Yi, Zeng Wei, Li Xin-hui, Su Jian-yu, Kou Ya-li, Jia Wen-xiang
Аннотация
OBJECTIVE: To probe the correlation between the ability of biofilm formation of Pseudomonas aeruginosa isolates and their genotypes.
METHODS: Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software.
RESULTS: Forty-eight Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters (A, B, C, D, E), were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level.
CONCLUSION: Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.
METHODS: Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software.
RESULTS: Forty-eight Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters (A, B, C, D, E), were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level.
CONCLUSION: Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.