[Quantification for biofilm formation of pseudomonas aeruginosa isolates and their genotypic analysis].

OBJECTIVE

To probe the correlation between the ability of biofilm formation of Pseudomonas aeruginosa isolates and their genotypes.

METHODS

Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software.

RESULTS

Forty-eight Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters (A, B, C, D, E), were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level.

CONCLUSION

Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.