Study on the anti-endotoxin effect of sinomenine using an Agilent genome array.

BACKGROUND

Endotoxin is a significant contributing factor underlying the occurrence of fever, diarrhea, inflammation, edema, coagulation, shock and other syndromes associated with gram-negative bacterial infections. To date, there is no effective treatment for endotoxemia.

AIM: The aim of this study was to characterize differentially expressed genes in sinomenine-treated and lipopolysaccharide (LPS)-induced endothelial cells by microarray analysis and to determine the potential pharmacological activity of sinomenine.

DESIGN

The cultured cells of five treatment groups (n = 3) were collected.

Participants

total RNA was extracted and subjected to Agilent Porcine 4 × 44 K whole genome microarray.

METHODS

Kyoto encyclopedia of genes and genomes and gene ontology software were applied to screen and analyze differentially regulated genes.

RESULTS

The results showed that 723 differentially regulated genes were identified including 410 up-regulated genes and 313 down-regulated genes in therapy group vs. LPS group. Ten genes may be key controlled genes in the pathogenesis of LPS, including five up-regulated genes (ARG1, TLR2, IL1A, VCAM1, DKK3) and five down-regulated genes (HABP2, ID1, CHDH, GPX3, PTGFR), which primarily contribute to biological processes such as inflammatory response, vascular lesion, metabolic process and cell cycle. IL1A and FMO3 were considered as potent target genes.

CONCLUSION

Global gene expression profile analysis showed that sinomenine might effectively be useful to regulate inflammatory responses as part of future anti-endotoxin therapies.