Cell reports methods · 2025
SpIC3D imaging for spinal in situ contrast 3D visualization.
СтатьяНазвание и аннотация: Перевод в очереди
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Liang Lucy, Fasse Alessandro, Damiani Arianna, Jantz Maria K, Agbor Uzoma, Del Brocco Matteo, Monney Rachel, Rowland Cecelia, Newton Taylor, Gopinath Chaitanya, Schaeffer David J, Kolarcik Christi L, Pagiazitis John G, Mentis George Z, Fisher Lee E, Neufeld Esra, Capogrosso Marco, Gaunt Robert A, Hitchens T Kevin, Pirondini Elvira
Аннотация
High-definition visualization techniques are critical for understanding the neuroanatomy of the spinal cord, an essential structure for sensorimotor and autonomic functions, in both healthy and pathological conditions. Magnetic resonance imaging (MRI) is a common method for visualizing neural structures in 3D. However, techniques for spinal cord MRI have historically achieved limited visualization of rootlets and nerves, especially at lower spinal levels, due to their highly complex and compact organization. Here, we developed a spinal in situ contrast 3D imaging (SpIC3D) method that allows visualization of spinal compartments in fixed animal and human specimens with high resolution (50 μm) at various spinal levels. Using SpIC3D, we achieved quantification of neuronal cell density in dorsal root ganglia, multi-segment identification of individual rootlets and roots, and volumetric reconstruction of multiple spinal structures for computational modeling. SpIC3D provides a basis for accelerated spinal pathology characterization and personalized spinal cord stimulation treatments.