Oncotarget · 2017
Regulatory T cells with a defect in inhibition on co-stimulation deteriorated primary biliary cholangitis.
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Chen Jianing, Hou Xianliang, Jia Hongyu, Cui Guangying, Wu Zhongwen, Wang Lin, Lu Chong, Wu Wei, Wei Yingfeng, Uede Toshimitsu, Li Lanjuan, Lian Zhexiong, Diao Hongyan
Аннотация
OBJECTIVES: Our aim was to evaluate protein levels of soluble inducible T-cell co-stimulator (ICOS) and membrane-bound ICOS on CD4+ and CD8+ T cells, and its ligand ICOSL on B cells and monocytes in newly diagnosed and established Sjögren's disease (SjD) patients, sicca, and healthy controls. Additionally, we aimed to investigate their associations with SjD clinical features and their diagnostic potential.
METHODS: We included 39 newly diagnosed SjD patients, 10 established SjD patients, 30 sicca controls and 27 healthy controls. Soluble ICOS levels were measured in serum using ELISA test and ICOS expression on CD4+ and CD8+ T cells and ICOSL expression on B cells and monocytes were measured in whole peripheral blood using flow cytometry.
RESULTS: Soluble ICOS and ICOS expressed on CD4+ T cells were significantly elevated in newly diagnosed SjD patients. Soluble ICOS showed a positive association with rheumatoid factor. ICOS expression on CD4+ T cells showed positive correlation with gamma globulins, and positive association with anti-Ro antibodies and rheumatoid factor. ROC curveanalysis revealed strong ability of soluble ICOS and ICOS expressed on CD4+ T cells to differentiate between SjD patients at the time they enter diagnostic process and sicca and healthy controls.
CONCLUSIONS: Soluble ICOS levels and ICOS expression on CD4+ T cells are elevated in SjD patients when they enter diagnostic process. Notably, this is the first study to report the high diagnostic potential of both markers, supporting their further evaluation as potential biomarkers in SjD.
METHODS: We included 39 newly diagnosed SjD patients, 10 established SjD patients, 30 sicca controls and 27 healthy controls. Soluble ICOS levels were measured in serum using ELISA test and ICOS expression on CD4+ and CD8+ T cells and ICOSL expression on B cells and monocytes were measured in whole peripheral blood using flow cytometry.
RESULTS: Soluble ICOS and ICOS expressed on CD4+ T cells were significantly elevated in newly diagnosed SjD patients. Soluble ICOS showed a positive association with rheumatoid factor. ICOS expression on CD4+ T cells showed positive correlation with gamma globulins, and positive association with anti-Ro antibodies and rheumatoid factor. ROC curveanalysis revealed strong ability of soluble ICOS and ICOS expressed on CD4+ T cells to differentiate between SjD patients at the time they enter diagnostic process and sicca and healthy controls.
CONCLUSIONS: Soluble ICOS levels and ICOS expression on CD4+ T cells are elevated in SjD patients when they enter diagnostic process. Notably, this is the first study to report the high diagnostic potential of both markers, supporting their further evaluation as potential biomarkers in SjD.