The Journal of antimicrobial chemotherapy · 2004
Identification of a chromosome-borne class C beta-lactamase from Erwinia rhapontici.
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Naas Thierry, Aubert Daniel, Vimont Sophie, Nordmann Patrice
Аннотация
OBJECTIVES: To characterize the beta-lactamase gene content of Erwinia rhapontici.
METHODS: The beta-lactamase gene was cloned, sequenced and expressed in Escherichia coli.
RESULTS: The cloned gene conferred a resistance pattern of an Ambler class C beta-lactamase in E. coli. The AmpC-type enzyme had a pI value of 8.6 and shared 62% amino acid sequence identity with that of Escherichia fergusonii. The ampC gene was associated with a regulatory ampR gene and beta-lactamase production was inducible.
CONCLUSIONS: This work provides further evidence of the molecular heterogeneity of beta-lactamases in Erwinia spp. and that plant-pathogenic enterobacterial species may constitute a reservoir of antibiotic resistance genes.
METHODS: The beta-lactamase gene was cloned, sequenced and expressed in Escherichia coli.
RESULTS: The cloned gene conferred a resistance pattern of an Ambler class C beta-lactamase in E. coli. The AmpC-type enzyme had a pI value of 8.6 and shared 62% amino acid sequence identity with that of Escherichia fergusonii. The ampC gene was associated with a regulatory ampR gene and beta-lactamase production was inducible.
CONCLUSIONS: This work provides further evidence of the molecular heterogeneity of beta-lactamases in Erwinia spp. and that plant-pathogenic enterobacterial species may constitute a reservoir of antibiotic resistance genes.