Zhongguo yi miao he mian yi · 2009
[Application of a simple method for detection of rubella virus genome by loop-mediated isothermal amplification (LAMP)].
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Wang Shuang, Zhou Jian-hui, Hou Xiang
Аннотация
OBJECTIVE: To use a new simple Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
METHOD: Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.
RESULTS: The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%.
CONCLUSION: RT-LAMP is more simple and convenient than RT-PCR.
METHOD: Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.
RESULTS: The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%.
CONCLUSION: RT-LAMP is more simple and convenient than RT-PCR.