[Construction of plasmid expression vector for specific peptide of the rubella virus E1 gene].

OBJECTIVE

To construct a recombinant plasmid vector of the RV specific fragment for expressing the specific fragment of RV E1 protein.

METHODS

RNA of the RV attenuated live vaccine Wistar RA27/3 strain was extracted and reversely transcribed. The specific fragment of the E1 gene was amplified and the PCR products cloned in the vector pGEX-2T after purification. Positive clones were selected and identified by two-enzyme digestion and sequence analysis.

RESULTS

A 330 bp target fragment was successfully cloned, and the sequence of the recombinant plasmid was consistent with the original sequence.

CONCLUSION

Successful cloning of the RV El specific fragment and the construction of the recombinant plasmid have laid a foundation for further expressing the recombinant protein.