[Expression of recombinant rubella virus E1 protein and initial application for detecting of antibody].

OBJECTIVE

To apply recombinant rubella virus envelope protein-1 (E1) to detect human rubella virus IgG antibody.

METHODS

Rubella virus E1 protein was expressed in E. coli, purified E1 protein was used as the antigen for the detecting of anti rubella in human sera in the way of enzyme linked Immunosorbant assay (ELISA).

RESULTS

The antigenicity of the recombinant protein was checked by WHO rubella sera panel. We detected 200 sera samples, which came from Guangxi Guilin. 93% of these samples were positive.

CONCLUSION

The antigenicity of recombinant E1 is a satisfied candidate antigen for the detecting of human rubella virus antibody. The prevalence of anti rubella virus IgG in Guangxi is 93%. It is at the some level compared with other provinces in China.