Biochemical and biophysical research communications · 2019
circRNA_0016624 could sponge miR-98 to regulate BMP2 expression in postmenopausal osteoporosis.
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Yu Long, Liu Yonggang
Аннотация
BACKGROUND: Circular RNAs (circRNAs) are emerging as important regulators in human disease. The expression profile and mechanism of circRNAs in postmenopausal osteoporosis remains largely unknown. Bone morphogenetic protein 2 (BMP2) is known to play important role in inducing osteogenic differentiation. MiR-98 was reported to regulate osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2.
AIM: We aimed to analyze circRNA expression profiles in osteoporosis and explore the molecular mechanism of circRNA_0016624 and interaction between circRNA_0016624, miR-98 and BMP2 during osteogenic differentiation.
METHODS: RNA-seq and bioinformatics analysis was performed in postmenopausal osteoporosis patients to screen for differentially expressed circRNAs. MiRanda and TargetScan were used to detect miR-98 binding sites of circRNA_0016624 and the target relationship was confirmed by dual luciferase assay. Expression level of circRNA_0016624, miR-98 and BMP2 were measured by qRT-PCR or Western blot. ARS staining was used to observe the level of osteogenic differentiation after transfection.
RESULTS: There were 387 circRNAs were differentially expressed in osteoporosis (|fold change| > 2 and P-value < 0.01). circRNA_0016624 and BMP2 were down-regulated in osteoporosis. CircRNA_0016624 could sponge miR-98 and regulate miR-98 expression. Overexpression of circRNA_0016624 promoted the expression of BMP2 and prevented osteoporosis.
CONCLUSION: circRNA_0016624 could sponge miR-98 and enhance BMP2 expression, thus circRNA_0016624 prevents osteoporosis and may provide a novel therapeutic strategy.
AIM: We aimed to analyze circRNA expression profiles in osteoporosis and explore the molecular mechanism of circRNA_0016624 and interaction between circRNA_0016624, miR-98 and BMP2 during osteogenic differentiation.
METHODS: RNA-seq and bioinformatics analysis was performed in postmenopausal osteoporosis patients to screen for differentially expressed circRNAs. MiRanda and TargetScan were used to detect miR-98 binding sites of circRNA_0016624 and the target relationship was confirmed by dual luciferase assay. Expression level of circRNA_0016624, miR-98 and BMP2 were measured by qRT-PCR or Western blot. ARS staining was used to observe the level of osteogenic differentiation after transfection.
RESULTS: There were 387 circRNAs were differentially expressed in osteoporosis (|fold change| > 2 and P-value < 0.01). circRNA_0016624 and BMP2 were down-regulated in osteoporosis. CircRNA_0016624 could sponge miR-98 and regulate miR-98 expression. Overexpression of circRNA_0016624 promoted the expression of BMP2 and prevented osteoporosis.
CONCLUSION: circRNA_0016624 could sponge miR-98 and enhance BMP2 expression, thus circRNA_0016624 prevents osteoporosis and may provide a novel therapeutic strategy.