Journal of veterinary science · 2026
East Asian Mycobacterium avium subsp. paratuberculosis: molecular typing and population structure.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Lee Jun Ho, Kyung Su Min, Lee Eun-Seo, Lee Han Gyu, Bok Eun-Yeong, Sreevatsan Srinand, Yoo Han Sang
Аннотация
IMPORTANCE: The global molecular epidemiology of Mycobacterium avium subsp. paratuberculosis (MAP) has been well documented in North America and Europe, but the population-genetic framework of the East Asian lineages is underrepresented, hindering a complete understanding of the global genotype distribution and transmission dynamics.
OBJECTIVE: This study aimed to establish a regional molecular-typing framework for MAP in East Asia and investigate its genetic relatedness with global lineages to explore potential intercontinental connections associated with international livestock movements.
METHODS: The study integrated 12 newly characterized MAP isolates from South Korean ruminant herds (cattle and black goats, 2021-2024) with 34 global isolates across six continents. Genotypic characterization was performed using mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) and multilocus short sequence repeat (MLSSR) approaches to evaluate the allelic diversity and subtyping resolution.
RESULTS: All South Korean isolates were identified as "cattle-type" (Type II) and predominantly belonged to the INRA Nouzilly MIRU-VNTR (INMV) genotype. Age-associated differences in MAP prevalence were observed among Korean ruminant herds (Fisher's exact test, p < 0.05), with distinct age-related patterns in beef and dairy cattle The integrated MIRU-VNTR and MLSSR approaches identified 14 and 31 distinct subtypes, respectively, with MLSSR showing superior discriminatory power (discriminatory index = 0.973 vs. 0.630 for MIRU-VNTR). This high allelic diversity in the global MLSSR dataset provided finer resolution than MIRU-VNTR for distinguishing the MAP subtypes across continents.
CONCLUSIONS AND RELEVANCE: This study provides a reference point for understanding the global diversity and distribution of MAP by bridging the genomic information gap in Asia. These findings underscore the potential of high-resolution, MLSSR-integrated surveillance as a practical tool for monitoring MAP across diverse geographical scales and for prioritizing isolates for future whole genome sequencing-based studies.
OBJECTIVE: This study aimed to establish a regional molecular-typing framework for MAP in East Asia and investigate its genetic relatedness with global lineages to explore potential intercontinental connections associated with international livestock movements.
METHODS: The study integrated 12 newly characterized MAP isolates from South Korean ruminant herds (cattle and black goats, 2021-2024) with 34 global isolates across six continents. Genotypic characterization was performed using mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) and multilocus short sequence repeat (MLSSR) approaches to evaluate the allelic diversity and subtyping resolution.
RESULTS: All South Korean isolates were identified as "cattle-type" (Type II) and predominantly belonged to the INRA Nouzilly MIRU-VNTR (INMV) genotype. Age-associated differences in MAP prevalence were observed among Korean ruminant herds (Fisher's exact test, p < 0.05), with distinct age-related patterns in beef and dairy cattle The integrated MIRU-VNTR and MLSSR approaches identified 14 and 31 distinct subtypes, respectively, with MLSSR showing superior discriminatory power (discriminatory index = 0.973 vs. 0.630 for MIRU-VNTR). This high allelic diversity in the global MLSSR dataset provided finer resolution than MIRU-VNTR for distinguishing the MAP subtypes across continents.
CONCLUSIONS AND RELEVANCE: This study provides a reference point for understanding the global diversity and distribution of MAP by bridging the genomic information gap in Asia. These findings underscore the potential of high-resolution, MLSSR-integrated surveillance as a practical tool for monitoring MAP across diverse geographical scales and for prioritizing isolates for future whole genome sequencing-based studies.