Biomaterials · 2023
Heart-derived collagen promotes maturation of engineered heart tissue.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Tani Hidenori, Kobayashi Eiji, Yagi Shinomi, Tanaka Keisuke, Kameda-Haga Kotaro, Shibata Shinsuke, Moritoki Nobuko, Takatsuna Kaworu, Moriwaki Taijun, Sekine Otoya, Umei Tomohiko C, Morita Yuika, Soma Yusuke, Kishino Yoshikazu, Kanazawa Hideaki, Fujita Jun, Hattori Shunji, Fukuda Keiichi, Tohyama Shugo
Аннотация
Although the extracellular matrix (ECM) plays essential roles in heart tissue engineering, the optimal ECM components for heart tissue organization have not previously been elucidated. Here, we focused on the main ECM component, fibrillar collagen, and analyzed the effects of collagens on heart tissue engineering, by comparing the use of porcine heart-derived collagen and other organ-derived collagens in generating engineered heart tissue (EHT). We demonstrate that heart-derived collagen induces better contraction and relaxation of human induced pluripotent stem cell-derived EHT (hiPSC-EHT) and that hiPSC-EHT with heart-derived collagen exhibit more mature profiles than those with collagens from other organs. Further, we found that collagen fibril formation and gel stiffness influence the contraction, relaxation, and maturation of hiPSC-EHT, suggesting the importance of collagen types III and type V, which are relatively abundant in the heart. Thus, we demonstrate the effectiveness of organ-specific collagens in tissue engineering and drug discovery.