Biomaterials advances · 2022
Self-assembled insulin-like growth factor 1 peptides induce adipose stem cell differentiation to repair cartilage injury.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wang Nan, Yang Jihao, Gan Guoli, Bao Xiaoyue, Wang Luyao
Аннотация
BACKGROUND: Tissue engineering using adipose-derived mesenchymal stem cells (ADSCs) promotes the regeneration of articular cartilage. However, insulin-like growth factor 1 (IGF-1), which is used to induce the differentiation of ADSCs into chondrocytes during treatment, is prone to instability and short tissue retention.
METHODS: Nap-FFG-GYGSSSRRAPQT was used as an IGF-1 mimicking molecule. MTT and CCK-8 assays were performed to evaluate the proliferation ability of ADSCs. QRT-PCR and Western blot assays were used to assess the expression of cartilage-related genes. International Cartilage Regeneration and Joint Preservation Society (ICRS) scoring was used for the evaluation of cartilage repair. Repaired tissues were analyzed by hematoxylin-eosin, Safranin-O and immunohistochemical staining.
RESULTS: Nap-FFG-GYGSSRRAPQT stimulated the proliferation and migration of ADSCs through the activation of IGF-1 receptor. Gel Nap-FFG-GYGSSRRAPQT treatment upregulated the expression of cartilage-related genes in ADSCs. ADSCs/Gel Nap-FFG-GYGSSRRAPQT treatment significantly promoted the regeneration of cartilages.
CONCLUSION: Self-assembled IGF-1 peptide, Nap-FFG-GYGSSRRAPQT, can induce ADSC differentiation and proliferation to repair cartilage injury.
METHODS: Nap-FFG-GYGSSSRRAPQT was used as an IGF-1 mimicking molecule. MTT and CCK-8 assays were performed to evaluate the proliferation ability of ADSCs. QRT-PCR and Western blot assays were used to assess the expression of cartilage-related genes. International Cartilage Regeneration and Joint Preservation Society (ICRS) scoring was used for the evaluation of cartilage repair. Repaired tissues were analyzed by hematoxylin-eosin, Safranin-O and immunohistochemical staining.
RESULTS: Nap-FFG-GYGSSRRAPQT stimulated the proliferation and migration of ADSCs through the activation of IGF-1 receptor. Gel Nap-FFG-GYGSSRRAPQT treatment upregulated the expression of cartilage-related genes in ADSCs. ADSCs/Gel Nap-FFG-GYGSSRRAPQT treatment significantly promoted the regeneration of cartilages.
CONCLUSION: Self-assembled IGF-1 peptide, Nap-FFG-GYGSSRRAPQT, can induce ADSC differentiation and proliferation to repair cartilage injury.