Epigenomics · 2016
Enlarged leukocyte referent libraries can explain additional variance in blood-based epigenome-wide association studies.
СтатьяПоддержка исследования: вне правительства СШАПоддержка NIH: внешняя программаНазвание и аннотация: Перевод в очереди
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Kim Stephanie, Eliot Melissa, Koestler Devin C, Houseman Eugene A, Wetmur James G, Wiencke John K, Kelsey Karl T
Аннотация
AIM: We examined whether variation in blood-based epigenome-wide association studies could be more completely explained by augmenting existing reference DNA methylation libraries.
MATERIALS & METHODS: We compared existing and enhanced libraries in predicting variability in three publicly available 450K methylation datasets that collected whole-blood samples. Models were fit separately to each CpG site and used to estimate the additional variability when adjustments for cell composition were made with each library.
RESULTS: Calculation of the mean difference in the CpG-specific residual sums of squares error between models for an arthritis, aging and metabolic syndrome dataset, indicated that an enhanced library explained significantly more variation across all three datasets (p < 10(-3)).
CONCLUSION: Pathologically important immune cell subtypes can explain important variability in epigenome-wide association studies done in blood.
MATERIALS & METHODS: We compared existing and enhanced libraries in predicting variability in three publicly available 450K methylation datasets that collected whole-blood samples. Models were fit separately to each CpG site and used to estimate the additional variability when adjustments for cell composition were made with each library.
RESULTS: Calculation of the mean difference in the CpG-specific residual sums of squares error between models for an arthritis, aging and metabolic syndrome dataset, indicated that an enhanced library explained significantly more variation across all three datasets (p < 10(-3)).
CONCLUSION: Pathologically important immune cell subtypes can explain important variability in epigenome-wide association studies done in blood.