Nature communications · 2021
Dissecting esophageal squamous-cell carcinoma ecosystem by single-cell transcriptomic analysis.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zhang Xiannian, Peng Linna, Luo Yingying, Zhang Shaosen, Pu Yang, Chen Yamei, Guo Wenjia, Yao Jiacheng, Shao Mingming, Fan Wenyi, Cui Qionghua, Xi Yiyi, Sun Yanxia, Niu Xiangjie, Zhao Xuan, Chen Liping, Wang Yuqian, Liu Yachen, Yang Xinyu, Wang Chengcheng, Zhong Ce, Tan Wen, Wang Jianbin, Wu Chen, Lin Dongxin
Аннотация
Esophageal squamous-cell carcinoma (ESCC), one of the most prevalent and lethal malignant disease, has a complex but unknown tumor ecosystem. Here, we investigate the composition of ESCC tumors based on 208,659 single-cell transcriptomes derived from 60 individuals. We identify 8 common expression programs from malignant epithelial cells and discover 42 cell types, including 26 immune cell and 16 nonimmune stromal cell subtypes in the tumor microenvironment (TME), and analyse the interactions between cancer cells and other cells and the interactions among different cell types in the TME. Moreover, we link the cancer cell transcriptomes to the somatic mutations and identify several markers significantly associated with patients' survival, which may be relevant to precision care of ESCC patients. These results reveal the immunosuppressive status in the ESCC TME and further our understanding of ESCC.
Предметные рубрики MeSH
AdultAgedB-LymphocytesEpithelial CellsEsophageal NeoplasmsEsophageal Squamous Cell CarcinomaFemaleFibroblastsGene Expression ProfilingGene Expression Regulation, NeoplasticHumansMaleMiddle AgedMyeloid CellsNeoplasm ProteinsPrognosisSingle-Cell AnalysisStromal CellsSurvival AnalysisT-LymphocytesTranscription, GeneticTumor MicroenvironmentWhole Genome Sequencing