Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment · 2012
Recent developments in molecular sub-typing of Listeria monocytogenes.
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Datta Atin R, Laksanalamai Pongpan, Solomotis Marianne
Аннотация
As a vast majority of the human listeriosis cases are caused by serotypes 1/2a, 1/2b and 4b strains, it is imperative that strains from clinical as well as from food and environment are further characterised so that accurate and timely epidemiological determination of sources of the contamination can be established to minimise the disease burden. Recent developments in the field of genomics provide a great opportunity to use these tools towards the development of molecular sub-typing techniques with a greater degree of discrimination spanning the entire length of the genome. This brief review summarises a few of these DNA-based techniques with an emphasis on DNA microarray and other whole genome sequencing-based approaches and their usefulness in Listeria monocytogenes sub-typing and outbreak investigations.