Diagnostic performances of Anyplex MTB/NTM Real-time detection vs Xpert® MTB/RIF Ultra for tuberculosis among people living with human immunodeficiency virus with low CD4 count.

OBJECTIVES

The aim of this study was to evaluate the performances of Anyplex® MTB/NTM Real-time Detection and Xpert MTB/RIF Ultra for tuberculosis (TB) disease in people living with Human Immunodeficiency Virus (PLHIV) with low CD4 + T lymphocyte cell (CD4) count.

PATIENTS AND

METHODS

A retrospective observational study included respiratory and non-respiratory specimens from PLHIV stratified based on their CD4 count (<200 vs. ≥ 200 cells/µL) using Anyplex® MTB/NTM Real-time Detection or Xpert® MTB/RIF Ultra.

RESULTS

In PLHIV with low CD4 count (<200 cells/µL), Anyplex demonstrated comparable sensitivity (81.4 % vs. 81.8 %) and specificity (78.7 % vs. 78.5 %) to Xpert for MTC detection in respiratory samples, but lower sensitivity (81.8 % vs. 100 %) and higher specificity (83.3 % vs. 66.6 %) than Xpert in non-respiratory samples.

CONCLUSIONS

Whereas the two methodologies showed similar MTC diagnostic performance in respiratory samples from PLHIV with low CD4 levels, Anyplex showed higher specificity and lower sensitivity than Xpert in non-respiratory samples.