Do zebrafish become blind or is it too much red dye in water? Distinguishing the embryo-larval development and physiology effects of DR 60, 73, and 78.

Some dyes currently used by the textile, pharmaceutical, food, cosmetic, and photographic industries have been shown to be toxic and/or mutagenic to aquatic life. Most of these dyes resist degradation processes available for treating wastewater, and these processes might generate even more toxic by-products. Despite the large number of available dyes and the large quantity of dyes released into the environment, studies on their toxicity are still scarce. We evaluated and compared the effects in the animal model Danio rerio (zebrafish) of environmentally relevant concentrations of Disperse Red 60 (DR 60), 73 (DR 73), and 78 (DR 78) using the fish embryo acute toxicity (FET) test, morphometric analysis, immunofluorescence imaging, and behavioral parameters. DR 60 caused ocular modifications, while the DR 73 caused non-inflation of the swim bladder (NISB), pericardial edema (PE), scoliosis (S) and abnormal yolk sac (AYS) from at 0.125 mg/L. In behavioral tests, all the dyes induced changes in velocity and time spent swimming of exposed larvae. However, these alterations in behavior seem to be caused by different factors dependent on the dye and its concentration. Nevertheless, behavior seems to add valuable information concerning the hazards analysis of dyes, since it reveals to be the most sensitive group of parameters tested in the current study. In conclusion, of the behavioral and developmental alterations caused by these dyes should be interpreted as an alert for greater attention when registering new dyes and releasing them into the environment. In the particular case of DR 60 the possibility that directly affects the eye of larvae is of great environmental concern, but also from the human health perspective.