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Войти в исследования Virus research · 2014
The haemagglutination activity of equine herpesvirus type 1 glycoprotein C.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Andoh Kiyohiko, Hattori Shiho, Mahmoud Hassan Y A H, Takasugi Maaya, Shimoda Hiroshi, Bannai Hiroshi, Tsujimura Koji, Matsumura Tomio, Kondo Takashi, Kirisawa Rikio, Mochizuki Masami, Maeda Ken
Аннотация
Equine herpesvirus type 1 (EHV-1) has haemagglutination (HA) activity toward equine red blood cells (RBCs), but the identity of its haemagglutinin is unknown. To identify the haemagglutinin of EHV-1, the major glycoproteins of EHV-1 were expressed in 293T cells, and the cells or cell lysates were mixed with equine RBCs. The results showed that only EHV-1 glycoprotein C (gC)-producing cells adsorbed equine RBCs, and that the lysate of EHV-1 gC-expressing cells agglutinated equine RBCs. EHV-1 lacking gC did not show HA activity. HA activity was inhibited by monoclonal antibodies (MAbs) specific for gC, but not by antibodies directed against other glycoproteins. In addition, HA activity was not inhibited by the addition of heparin. These results indicate that EHV-1 gC can bind equine RBCs irrespective of heparin, in contrast to other herpesvirus gC proteins.