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Войти в исследования Tuberculosis (Edinburgh, Scotland) · 2025
Exploring CHIT1 and YKL-40 in tuberculous pleural effusion: Insights and implications.
СтатьяНаблюдательное исследованиеНазвание и аннотация: Перевод в очереди
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Przysucha Natalia, Paplińska-Goryca Magdalena, Górska Katarzyna, Misiukiewicz-Stępień Paulina, Mlącki Michał, Cyran Agata, Krenke Rafal
Аннотация
BACKGROUND: Chitinases and chitinase-like proteins are implicated in the pathophysiology of lung diseases. This study aimed to evaluate the significance of chitotriosidase (CHIT1) and YKL-40 in tuberculous pleural effusion (TPE), identify their cellular sources, and assess their diagnostic potential as TPE biomarkers.
METHODS: This observational, retrospective study included 66 patients with pleural effusion of different origins: malignant (MPE), tuberculous (TPE), parapneumonic (PPE), and transudative (TE). Pleural fluid levels of YKL-40 and CHIT1 were measured. Expressions of YKL-40 and CHIT1 in tuberculous pleural granulomas were also assessed using immunohistochemical staining.
RESULTS: We found the highest median CHIT1 and YKL-40 levels for TPE: 70.51 (interquartile range [IQR] 49.65-136.98) ng/mL and 569.84 (IQR 530.32-706.01) ng/mL, respectively. YKL-40 was significantly higher in TPE than in PPE (387.98 [IQR 262.94-539.09] ng/mL, p < 0.01)] and TE (254.95 [IQR 188.93-334.1 ng/ml] ng/mL, p < 0.001). There was a strong positive correlation between the YKL-40 level in TPE and the percentage of macrophages (r = 0.73, p = 0.003) and the adenosine deaminase activity (r = 0.82, p < 0.001). We revealed strong YKL-40 expression in tuberculoid pleural granulomas.
CONCLUSION: YKL-40, but not CHIT-1, may contribute to the pleural inflammatory response associated with tuberculosis.
METHODS: This observational, retrospective study included 66 patients with pleural effusion of different origins: malignant (MPE), tuberculous (TPE), parapneumonic (PPE), and transudative (TE). Pleural fluid levels of YKL-40 and CHIT1 were measured. Expressions of YKL-40 and CHIT1 in tuberculous pleural granulomas were also assessed using immunohistochemical staining.
RESULTS: We found the highest median CHIT1 and YKL-40 levels for TPE: 70.51 (interquartile range [IQR] 49.65-136.98) ng/mL and 569.84 (IQR 530.32-706.01) ng/mL, respectively. YKL-40 was significantly higher in TPE than in PPE (387.98 [IQR 262.94-539.09] ng/mL, p < 0.01)] and TE (254.95 [IQR 188.93-334.1 ng/ml] ng/mL, p < 0.001). There was a strong positive correlation between the YKL-40 level in TPE and the percentage of macrophages (r = 0.73, p = 0.003) and the adenosine deaminase activity (r = 0.82, p < 0.001). We revealed strong YKL-40 expression in tuberculoid pleural granulomas.
CONCLUSION: YKL-40, but not CHIT-1, may contribute to the pleural inflammatory response associated with tuberculosis.