Proportion of Immunoregulatory Cells in Inflammatory Infiltrate in Rheumatoid Arthritis Model and Its Correction by Antisense Oligonucleotides.

Autoimmune inflammation in rheumatoid arthritis is caused by various immunocompetent cells secreting a wide range of cytokines. Macrophages, CD4+, and CD8+ T cells are the most active in this process. We propose using antisense oligonucleotides (ASO) that block the synthesis of TNFα and IL-6. In the adjuvant-induced arthritis model, changes in the number of CD4+, CD8+, and CD68+ cells in the inflammatory infiltrate after correction with ASO were assessed. The number of immunopositive inflammatory infiltrate cells in the area of bone damage was counted. CD8+ cells turned out to be the most numerous. Blockade of IL-6 led to a statistically significant decrease in the number of CD4+ cells. The use of ASO reduced the number of macrophages. The combination of ASO against IL-6 and TNFα administered by electrophoresis most effective.