Ascorbic Acid Modulates Collagen Properties in Glucocorticoid-Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation.

Osteoporosis is a prevalent skeletal disorder characterized by decreased bone mass and structural deterioration, leading to an increased risk of fractures. This study focuses on the regulatory role of Vitamin C (ascorbic acid; AA) in the context of glucocorticoid-induced osteoporosis (GIOP), which results from long-term glucocorticoid (GC) therapy. The data showed that GCs impair AA metabolism in osteoblasts, thereby disrupting collagen synthesis and compromising extracellular matrix (ECM) integrity. Notably, AA integration in the collagen matrix improved its biochemical and mechanical properties. Additionally, it has been shown that the presence of AA restored osteoblast and endothelial function, enhanced collagen production, and improved endothelial barrier function under GC exposure. These results underscore the critical role of Vitamin C in bone matrix maintenance and homeostasis. Collectively, this work highlights the therapeutic potential of Vitamin C as a supportive treatment to counteract the deleterious skeletal effects of long-term GC therapy.