Comparative longitudinal analysis of T lymphocyte subpopulations in calves vaccinated with different doses of BCG-Phipps or with culture filtrate protein extract of Mycobacterium bovis in a natural transmission setting.

Currently, control programmes for bovine tuberculosis (bTB) contemplate the use of vaccines to reduce disease incidence rates. The BCG vaccine and the culture filtrate protein extract (CFPE) of Mycobacterium bovis are strong candidates for vaccination against bTB. We conducted an analysis of the immune response and evaluated activation and memory markers in CD4+ and CD8+ T-lymphocyte subpopulations in Holstein-Friesian calves immunised with different doses of M. bovis BCG-Phipps vaccine (1×10⁴ and 1×10⁶ CFU) or with CFPE (300 µg and 600 µg) in a natural transmission setting. The study was carried out in a dairy herd, selecting calves aged 1-4 months that tested negative in various bTB diagnostic tests. In the groups immunised with the BCG-Phipps vaccine, gamma interferon (IFN-γ) secretion levels increased significantly, with the highest increase observed in the group that received a dose of 1×10⁶ CFU (P ≤ 0.05). The CD4+/CD8+ ratio increased significantly over time in both vaccinated and unvaccinated groups, with no significant differences between them. However, notable differences were observed in activated (CD25+) and memory (CD45RO+) CD4 and CD8 T-cell populations across different times and treatments. Remarkably, the groups immunised with the BCG vaccine remained free of M. bovis infection, as evidenced by negative IFN-γ results using ESAT-6/CFP-10 antigens and negative PCR test results for bacterial detection. The comparative analysis of the immune response induced by the different doses of the BCG-Phipps and CFPE vaccines revealed that the group of animals vaccinated with the 1×10⁶ CFU dose exhibited greater production of gamma interferon and a higher percentage of CD4+ T cells, as well as activated and memory CD8+ T cells compared to the other vaccinated and control groups in the natural transmission environment.