circAGO3 facilitates NF-κB pathway-mediated inflammatory atrophy in chicken skeletal muscle via the miR-34b-5p/TRAF3 axis.

Circular RNAs have emerged as critical regulators of gene expression across various biological systems. In this study, circAGO3, originating from exons 5, 6, 7, and 8 of the AGO3 gene in chickens, is characterized for its stability and differential expression during both embryonic and post-hatch stages. Overexpression of circAGO3 in chicken skeletal muscle markedly disrupts myogenesis by downregulating muscle differentiation markers and upregulating genes associated with muscle atrophy. RNA sequencing and functional analyses further delineate circAGO3's involvement in modulating inflammatory responses through the NF-κB signaling pathway, mediated by its interaction with miR-34b-5p. These findings highlight circAGO3's potential importance in poultry production by uncovering its regulatory roles in skeletal muscle development and inflammation, positioning it as a promising target for enhancing muscle growth and health in poultry farming.