Veterinary clinical pathology · 2026
Life-Stage-Specific Hematological Profiles in Yellow Mealworm Beetles (Tenebrio molitor): Reference Intervals and Hemocyte Characterization.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Maheu Simon, Ben-Miled Houda, Marullo-Masson Dominique, Favret Colin, Gara-Boivin Carolyn, Benoit-Biancamano Marie-Odile
Аннотация
BACKGROUND: The edible insect industry, driven by the nutritional and environmental benefits of mealworms, faces increasing challenges from pathogens affecting Tenebrio molitor farming systems. Hemocyte-based health assessments could provide valuable diagnostic insights, yet standardized hemolymph processing protocols and hematological reference intervals (RIs) are currently lacking for this species.
OBJECTIVES: This study aimed to develop practical protocols for hemolymph sampling, refine hemocyte classification, and establish hematological RIs for both larval and adult stages of T. molitor.
METHODS: An optimized tearing method, involving capillary collection of hemolymph from a proleg transected at the femoral-tibial joint, was used to sample healthy individuals. Total and differential hemocyte counts were determined using a hemocytometer and Wright-Giemsa-stained smears, respectively, and from distinct insect populations (n = 41-43 samples per group). Hemolymph was analyzed in pools due to the small size of T. molitor. Transmission electron microscopy was conducted on a multinucleated giant hemocyte (MGH) isolated from a histologic slide. RIs were calculated following American Society for Veterinary Clinical Pathology (ASVCP) guidelines, and life-stage differences were statistically assessed.
RESULTS: Granular cells and plasmatocytes predominated, whereas prohemocytes and oenocytoids were rare. MGHs, newly identified in T. molitor, were characterized as a fifth hemocyte population. Significant life-stage differences were observed, warranting separate RIs for larvae and adults. The established RIs were relatively wide, reflecting natural fluctuations in invertebrate hemolymph volume and the unsexed, age-diverse reference population.
CONCLUSIONS: This report of hematological RIs for T. molitor lays the groundwork for implementing hemocyte-based health surveillance in yellow mealworm beetle farming.
OBJECTIVES: This study aimed to develop practical protocols for hemolymph sampling, refine hemocyte classification, and establish hematological RIs for both larval and adult stages of T. molitor.
METHODS: An optimized tearing method, involving capillary collection of hemolymph from a proleg transected at the femoral-tibial joint, was used to sample healthy individuals. Total and differential hemocyte counts were determined using a hemocytometer and Wright-Giemsa-stained smears, respectively, and from distinct insect populations (n = 41-43 samples per group). Hemolymph was analyzed in pools due to the small size of T. molitor. Transmission electron microscopy was conducted on a multinucleated giant hemocyte (MGH) isolated from a histologic slide. RIs were calculated following American Society for Veterinary Clinical Pathology (ASVCP) guidelines, and life-stage differences were statistically assessed.
RESULTS: Granular cells and plasmatocytes predominated, whereas prohemocytes and oenocytoids were rare. MGHs, newly identified in T. molitor, were characterized as a fifth hemocyte population. Significant life-stage differences were observed, warranting separate RIs for larvae and adults. The established RIs were relatively wide, reflecting natural fluctuations in invertebrate hemolymph volume and the unsexed, age-diverse reference population.
CONCLUSIONS: This report of hematological RIs for T. molitor lays the groundwork for implementing hemocyte-based health surveillance in yellow mealworm beetle farming.