Physical therapy in sport : official journal of the Association of Chartered Physiotherapists in Sports Medicine · 2016
Pulsed electromagnetic field ameliorates cartilage degeneration by inhibiting mitogen-activated protein kinases in a rat model of osteoarthritis.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhou Jun, Liao Yuan, Xie Haitao, Liao Ying, Liu Huifang, Zeng Yahua, Li Neng
Аннотация
OBJECTIVES: We assessed the effects of pulsed electromagnetic field (PEMF) on cartilage degeneration, and expression of mitogen-activated protein kinases (MAPKs) and matrix metalloproteinases (MMPs), in an experimental rat model of osteoarthritis induced by anterior cruciate ligament transection (ACLT).
DESIGN: Experimental.
SETTING: University animal laboratory.
PARTICIPANTS: 30 male Sprague-Dawley rats.
MAIN OUTCOME MEASURES: We performed histological examination, enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, to assess cartilage degeneration, urine C-terminal cross-linking telopeptide of type II collagen (CTX-II), and mRNA expression of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (c-Jun), p38, and MMPs.
RESULTS: Urinary CTX-II in the PEMF group was significantly lower than in the ACLT group at 9 and 13 weeks. Mankin scores in the PEMF group significantly lower than that in the ACLT group (P < 0.01). mRNA expression of ERK1, c-Jun, p38, MMP-13 and MMP-3 was significantly higher in the ACLT group than in the Sham group, while that with the sole exception of MMP-3 in the PEMF group was significantly lower than in the ACLT group.
CONCLUSIONS: PEMF may regulate the catabolic factor, MMP13, and inhibit cartilage destruction, at least partially, by inhibiting MAPKs signaling pathway.
DESIGN: Experimental.
SETTING: University animal laboratory.
PARTICIPANTS: 30 male Sprague-Dawley rats.
MAIN OUTCOME MEASURES: We performed histological examination, enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, to assess cartilage degeneration, urine C-terminal cross-linking telopeptide of type II collagen (CTX-II), and mRNA expression of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (c-Jun), p38, and MMPs.
RESULTS: Urinary CTX-II in the PEMF group was significantly lower than in the ACLT group at 9 and 13 weeks. Mankin scores in the PEMF group significantly lower than that in the ACLT group (P < 0.01). mRNA expression of ERK1, c-Jun, p38, MMP-13 and MMP-3 was significantly higher in the ACLT group than in the Sham group, while that with the sole exception of MMP-3 in the PEMF group was significantly lower than in the ACLT group.
CONCLUSIONS: PEMF may regulate the catabolic factor, MMP13, and inhibit cartilage destruction, at least partially, by inhibiting MAPKs signaling pathway.
Предметные рубрики MeSH
AnimalsCartilage, ArticularCollagen Type IIDisease Models, AnimalElectromagnetic FieldsEnzyme-Linked Immunosorbent AssayExtracellular Signal-Regulated MAP KinasesJNK Mitogen-Activated Protein KinasesMAP Kinase Signaling SystemMaleMatrix Metalloproteinase 13Mitogen-Activated Protein KinasesOsteoarthritisPeptide FragmentsRNA, MessengerRatsRats, Sprague-DawleyReal-Time Polymerase Chain Reaction