Ultraviolet C lethal effect on Brucella melitensis.

The gram-negative bacteria Brucella melitensis was investigated to evaluate its susceptibility to UVC radiation at 254 nm. At an intensity of 18.7 mW/cm2 of UVC, the time required for inactivation of B. melitensis was 240 seconds in both dark and light, whereas it was 120 seconds and 240 seconds in dark and light respectively at an intensity of 19.5 mW/cm2. The results indicate that vaccinal strain of B. melitensis (Rev.1) is more sensitive to UVC than wild B. melitensis strain.