[Role of Ginkgo biloba extract in regulating 5-hydroxytrytamine and its receptor in heart failure mice].

Objective

Exploring the modulation of periphery and central serotonin (5-hydroxytrytamine, 5-HT) and 5-HT(2A) receptor (5-HT(2A)R) in mice with heart failure during the process of heart failure and after treatment.

Methods

Thirty mice were allocated into three groups randomly including normal group (NG), heart failure group (HFG) and treatment group (TG) (n=10). Mice in the NG were sacrificed after evaluating left ventricular ejection fraction (EF) with echocardiogram. Mice in HFG and TG experienced myocardial infarction surgery to achieve EF≤30%, HFG was treated with saline and TG with Ginkgo biloba pills (GBP), once a day for 4 weeks, then EF was assessed again before sacrifice. Cardiac muscle tissue fibrosis was observed and the severity of fibrosis was assessed by semiquantitative method. All the indexes including BNP, serum 5-HT, 5-HT and 5-HT(2A)R in platelet and brain were detected. The data were analyzed by SPSS 19.0.

Results

EF: EF in HFG and TG were much lower than that in NG (P<0.05), TG was much higher than HFG (P<0.05). BNP: BNP in HFG and TG were much higher than that in NG (P<0.05), TG was significantly lower than HFG (P<0.05). HFG showed ventricular remodeling, increased myocardial fibrosis; while myocardial fibrosis decreased in TG. 5-HT in serum: serum 5-HT in HFG was much higher than NG (212±29 ng/L vs 146±5 ng/L, P<0.05), TG was much lower than HFG (163±8 ng/L vs 212±29 ng/L, P<0.05). 5-HT in platelet: platelet 5-HT in HFG was much lower than NG (165±22 ng/L vs 193±19 ng/L, P<0.05), platelet 5-HT in TC was higher than HFG and NG (224±27 ng/L vs 193±19 ng/L, 165±22 ng/L, P<0.05). 5-HT(2A)R in platelet: platelet 5-HT(2A)R in HFG was much lower than NG (P<0.05), TG was much higher than NG and HFG (P<0.05). 5-HT in cortex: cortex 5-HT in HFG and TG were much higher than NG (253±4 ng/L, 231±31 ng/L vs 179±9 ng/L, P<0.05), TG was a little lower than HFG (231±31 ng/L vs 253±4 ng/L, P>0.05). 5-HT(2A)R in cortex: cortex 5-HT(2A)R in HFG and TG were much higher than NG (P<0.05), TG was much lower than HFG (P<0.05). 5-HT in hippocampus: hippocampus 5-HT in HFG was much higher than NG (250±7 ng/L vs 179±5 ng/L, P<0.05), TG was much lower than HFG (164±6 ng/L vs 250±7 ng/L, P<0.05). 5-HT(2A)R in hippocampus: hippocampus 5-HT in both HFG and TG were much higher than NG (P<0.05), there was no significant difference between TG and HFG (P>0.05).

Conclusion

Significant changes of 5-HT and 5-HT(2A)R are found in heart failure mice during the process of heart failure and after treatment, indicating the potential value of 5-HT modulation in the diagnose and treatment of heart failure.