Journal of the American Heart Association · 2016
Pretreatment of Cardiac Stem Cells With Exosomes Derived From Mesenchymal Stem Cells Enhances Myocardial Repair.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zhang Zhiwei, Yang Junjie, Yan Weiya, Li Yangxin, Shen Zhenya, Asahara Takayuki
Аннотация
BACKGROUND: Exosomes derived from mesenchymal stem cells (MSCs) were proved to boost cell proliferation and angiogenic potency. We explored whether cardiac stem cells (CSCs) preconditioned with MSC exosomes could survive and function better in a myocardial infarction model.
METHODS AND RESULTS: DiI-labeled exosomes were internalized with CSCs. They stimulated proliferation, migration, and angiotube formation of CSCs in a dose-dependent manner. In a rat myocardial infarction model, MSC exosome-preconditioned CSCs had significantly better survival, enhanced capillary density, reduced cardiac fibrosis, and restored long-term cardiac function. MicroRNA profiling analysis revealed that a set of microRNAs were significantly changed in CSCs after MSC exosome treatment.
CONCLUSIONS: Pretreatment of CSCs with MSC exosomes provided a promising strategy to improve survival and angiogenic potency of CSCs.
METHODS AND RESULTS: DiI-labeled exosomes were internalized with CSCs. They stimulated proliferation, migration, and angiotube formation of CSCs in a dose-dependent manner. In a rat myocardial infarction model, MSC exosome-preconditioned CSCs had significantly better survival, enhanced capillary density, reduced cardiac fibrosis, and restored long-term cardiac function. MicroRNA profiling analysis revealed that a set of microRNAs were significantly changed in CSCs after MSC exosome treatment.
CONCLUSIONS: Pretreatment of CSCs with MSC exosomes provided a promising strategy to improve survival and angiogenic potency of CSCs.
Предметные рубрики MeSH
AnimalsAnimals, NewbornCell DifferentiationCell MovementCell ProliferationCells, CulturedDisease Models, AnimalEndothelial Progenitor CellsExosomesFibrosisGene Expression RegulationGene Regulatory NetworksMesenchymal Stem CellsMicroRNAsMyocardial InfarctionMyocardiumMyocytes, CardiacNeovascularization, PhysiologicPhenotypeRats, Sprague-DawleyRecovery of FunctionRegenerationSignal TransductionStem Cell TransplantationStroke VolumeTime FactorsVentricular Function, Left