[Regulatory effect of curcumin on subsets and co-stimulatory molecules of dendritic cells in spleen from mice with colitis].

OBJECTIVE

To explore the effect of curcumin (Cur) on the subpopulation and costimulatory molecules of dendritic cells (DCs) in spleen from colitis mice.

METHODS

Forty male C57/BL mice were randomly divided into 4 groups: normal group, model group, Cur group and mesalazine group. Colitis was induced by 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). On the 8th day following 7-day treatment with Cur, the mice were sacrificed and the length and mass of mouse colon were measured. And then the spleens were separated to prepare DCs. The numbers of CD205(+), CD4(+)CD205(+), CD8(+)CD205(+) DCs and the levels of major histocompability complex II (MHC-II), Toll-like receptor 2 (TLR2), TLR4 and CD83 expression were detected by flow cytometry. And we observed the pathological injury of colon.

RESULTS

Compared with model group, the colonic mass index was reduced, the length of colon was lengthened, and the pathological injury was remitted. Meanwhile, the percents of CD205(+), CD4(+)CD205(+), CD8(+)CD205(+) DCs and the levels of MHC-II, TLR2 and TLR4 were down-regulated, however, the CD83 expression was up-regulated.

CONCLUSION

Treatment efficacy of Cur on experimental colitis might be involved in regulating subpopulation and co-stimulatory molecules of dendritic cells in spleen from mice with colitis.