[Comparison of different sampling procedures in negative pressure wound therapy used in wound infections following reconstruction of abdominal wall hernias with mesh on homogeneous patient material].

INTRODUCTION: The repair of postoperative abdominal hernias remains a challenge to this day, even though the surgery is sterile. Wound infection is a problem, which is exacerbated by the comorbidity of the patient population. The incorporation of foreign material always carries a risk of infection. In the event of wound infection, the possibility of hernia recurrence is 0.2-8%, while if the mesh also needs to be removed, the probability of recurrence is over 30%. Compared to open wound treatment, negative pressure therapy is more effective in promoting wound healing and preserving the implanted mesh.

MATERIALS AND METHODS: In our study, we analyze the cases of a total of 30 patients who underwent onlay mesh abdominal hernia surgery between 2015 and 2023, during which wound dehiscence occurred. In all cases, wound exudate culture and negative pressure wound therapy were performed. In 15 patients, swab samples were taken, while in the other 15, swab, sponge, and tissue roller samples were taken. Swab samples mainly revealed skin flora, which was Gram-positive in 71% of cases. The results of the tissue roller and sponge swab cultures showed Gram-negative pathogens in 50% of cases.

RESULTS: The sponge swab and tissue roller cultures provided additional information on the bacterial load. Negative pressure wound therapy meant that the mesh did not need to be removed.

CONCLUSION: During negative pressure wound therapy, cultures taken using multiple methods also helped in the selection of antibiotics. Vacuum therapy used for mesh removal reduces bacterial load and accelerates granulation. Hernia recurrence and mesh removal are significantly reduced. Orv Hetil. 2026; 167(18): 714-721.