Swadamshtradi rasayana attenuates radiofrequency radiation-induced oxidative stress and improves sperm parameters in Swiss albino mice.

INTRODUCTION

Exposure to radiofrequency electromagnetic radiation (RF-EMR) has been linked in several studies to male reproductive dysfunction, with oxidative stress and sperm DNA damage proposed as key underlying mechanisms.

METHODS

The present study evaluated the protective effect of Swadamshtradi rasayana (SDR) against RF-EMR-induced sperm alterations in mice. Twenty-four Swiss albino mice were allocated into four groups: control, RF-EMR-exposed, RF-EMR with Swadamshtradi rasayana (729 mg/kg, orally), and RF-EMR with α-tocopherol (32.4 mg/kg, orally). Animals were exposed to RF-EMR (900-1800MHz) for 3 h/day for 35 days, and sperm count, viability, reactive oxygen species levels, antioxidant enzyme activity, lipid peroxidation, and DNA fragmentation were assessed.

RESULTS

RF-EMR exposure was associated with a significant reduction in sperm count and viability and a significant increase in ROS levels, lipid peroxidation, and DNA fragmentation (p < 0.0001 compared with control). Administration of Swadamshtradi rasayana significantly improved sperm parameters and attenuated oxidative stress compared with the RF-EMR-exposed group (p < 0.0001).

CONCLUSION

These findings suggest that Swadamshtradi rasayana may mitigate RF-EMR-induced oxidative stress and associated sperm damage. The observed protective effects are consistent with the reported antioxidant properties of its constituent herbs.