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Войти в исследования Journal of investigative surgery : the official journal of the Academy of Surgical Research · 2026
Impact of PA-MSHA on Postoperative Seroma Formation and Tissue Remodeling in Rat Abdominal Wall Hernia Model.
СтатьяНазвание и аннотация: Перевод в очереди
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Guo Wenke, Fei Bin, Niu Haigang, Shi Chuya, Ren Yaoqiang, Ren Xia, Liu Xinrui
Аннотация
BACKGROUND: Seroma is one of the most common postoperative complications following hernia repair. This study evaluated whether the local application of Pseudomonas aeruginosa injection (PA-MSHA) could reduce the duration of seroma and promote healing of the graft and surrounding tissues after abdominal wall incisional hernia repair.
METHODS: 56 Sprague-Dawley rats were used to assess the efficacy and optimal dosage of PA-MSHA in reducing seroma formation after latissimus dorsi resection. 54 rats underwent abdominal wall hernia repair and were randomly assigned to three groups: sham, saline, or PA-MSHA. Seroma formation was monitored, and tissue samples were collected for histological and immunohistochemical analyses.
RESULTS: Among five groups, the medium-dose group (0.1 mL/kg) demonstrated the most significant reduction in seroma volume. PA-MSHA significantly shortened the duration of seroma and enhanced both early and mid-term tissue healing. Sirius Red staining indicated a significantly higher collagen type I/III ratio on days 7 and 14. Immunohistochemical analysis demonstrated elevated expression of CD68 and CD31 during the early healing phase in the PA-MSHA group. By day 28, graft shrinkage did not differ significantly among the groups.
CONCLUSIONS: Intraoperative local administration of PA-MSHA effectively reduced seroma persistence and enhanced early tissue regeneration and abdominal wall integrity without triggering excessive foreign body reactions or adverse events.
METHODS: 56 Sprague-Dawley rats were used to assess the efficacy and optimal dosage of PA-MSHA in reducing seroma formation after latissimus dorsi resection. 54 rats underwent abdominal wall hernia repair and were randomly assigned to three groups: sham, saline, or PA-MSHA. Seroma formation was monitored, and tissue samples were collected for histological and immunohistochemical analyses.
RESULTS: Among five groups, the medium-dose group (0.1 mL/kg) demonstrated the most significant reduction in seroma volume. PA-MSHA significantly shortened the duration of seroma and enhanced both early and mid-term tissue healing. Sirius Red staining indicated a significantly higher collagen type I/III ratio on days 7 and 14. Immunohistochemical analysis demonstrated elevated expression of CD68 and CD31 during the early healing phase in the PA-MSHA group. By day 28, graft shrinkage did not differ significantly among the groups.
CONCLUSIONS: Intraoperative local administration of PA-MSHA effectively reduced seroma persistence and enhanced early tissue regeneration and abdominal wall integrity without triggering excessive foreign body reactions or adverse events.