[A study on expression of hsp70 mRNA in the early stage of diffuse brain injury in rats].

OBJECTIVE

To evaluate the changes of hsp70 mRNA in neuron and glia of rat suffering early diffuse brain injury.

METHODS

SD rats were used in the replication of the animal pattern of diffuse brain injury. In situ hybridization technique and image analysis technique were applied in detecting the hsp70 mRNA in the rat's cerebral cortex, thalamus and brain stem.

RESULTS

The signal intensity of hsp70 mRNA could be detected in 5 minutes after brain injury; subsequently, it attained to the summit at 6 hours and declined at 12 hours after brain injury.

CONCLUSION

The above data suggest that hsp70 mRNA is useful for the diagnosis of early diffuse brain injury and for distinguishing the postmortem injury from antemortem injury.