Journal of ethnopharmacology · 2026
The protective effects of phenolic-rich breadfruit (Artocarpus altilis) ethanol extract against trichloroethylene-induced nephrotoxicity: In vivo and in silico studies.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Dahunsi Damilola Timothy, Lawal Akeem Olalekan, Akinjiyan Moses Orimoloye, Agboola Olaoluwa Oladimeji, Ijatuyi Taiwo Tolulope, Ademosun Ayokunle Olubode, Remilekun Akinrinmade
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Breadfruit (Artocarpus altilis) has been traditionally used as a nephroprotective agent, but limited information exists on its underlying molecular mechanisms. Trichloroethylene (TCEL) is a toxicant that negatively impacts the kidneys.
AIM OF THE STUDY: This study investigated breadfruit leaf ethanol extract's (BF) ability to protect against TCEL-induced nephrotoxicity. Thirty Wistar rats were divided into six groups (n = 5) and treated orally for 15 days. Group I received corn oil; group II (TCEL 1000 mg/kg); groups III and IV received BF at 100 and 200 mg/kg, respectively. Groups V and VI were administered 100 and 200 mg/kg of BF, respectively, 2 h before TCEL exposure. Serum urea, albumin, uric acid, creatinine, and bilirubin were evaluated using standard protocols after sacrifice. KIM-1, SOD, CAT, JNK, p38 MAPK, and p53 genes' expression studies were done with RT-PCR. Molecular docking and free binding energy of BF compounds with NF-κB, p38 MAPK, and p53 were performed using the Schrödinger suite.
RESULTS: TCEL (1000 mg/kg) significantly (p < 0.05) elevated urea, albumin, uric acid, creatinine, and bilirubin serum levels and upregulated KIM-1 and JNK gene expression compared to control. Pre-treatment of rats with BF (100 and 200 mg/kg) significantly increased SOD and CAT gene expressions, reduced serum levels of kidney biomarkers, and modulated p38 MAPK and p53 gene expressions compared with TCEL-induced untreated groups. The free binding energies and molecular docking of A. altilis compounds with NF-κB, p38 MAPK, and p53 suggest possible interactions.
CONCLUSION: The study suggests that BF could be an effective agent against TCEL-induced toxicity.
AIM OF THE STUDY: This study investigated breadfruit leaf ethanol extract's (BF) ability to protect against TCEL-induced nephrotoxicity. Thirty Wistar rats were divided into six groups (n = 5) and treated orally for 15 days. Group I received corn oil; group II (TCEL 1000 mg/kg); groups III and IV received BF at 100 and 200 mg/kg, respectively. Groups V and VI were administered 100 and 200 mg/kg of BF, respectively, 2 h before TCEL exposure. Serum urea, albumin, uric acid, creatinine, and bilirubin were evaluated using standard protocols after sacrifice. KIM-1, SOD, CAT, JNK, p38 MAPK, and p53 genes' expression studies were done with RT-PCR. Molecular docking and free binding energy of BF compounds with NF-κB, p38 MAPK, and p53 were performed using the Schrödinger suite.
RESULTS: TCEL (1000 mg/kg) significantly (p < 0.05) elevated urea, albumin, uric acid, creatinine, and bilirubin serum levels and upregulated KIM-1 and JNK gene expression compared to control. Pre-treatment of rats with BF (100 and 200 mg/kg) significantly increased SOD and CAT gene expressions, reduced serum levels of kidney biomarkers, and modulated p38 MAPK and p53 gene expressions compared with TCEL-induced untreated groups. The free binding energies and molecular docking of A. altilis compounds with NF-κB, p38 MAPK, and p53 suggest possible interactions.
CONCLUSION: The study suggests that BF could be an effective agent against TCEL-induced toxicity.