Performance and Diagnostic Value of Genome-Wide Noninvasive Prenatal Testing in Multiple Gestations.
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van Riel Margot, Brison Nathalie, Baetens Machteld, Blaumeiser Bettina, Boemer François, Bourlard Laura, Bulk Saskia, De Leener Anne, Désir Julie, Devriendt Koenraad, Dheedene Annelies, Duquenne Armelle, Fieremans Nathalie, Fieuw Annelies, Gatot Jean-Stéphane, Grisart Bernard, Janssens Sandra, Khudashvili Naïri, Lannoo Lore, Marichal Axel, Meunier Colombine, Palmeira Leonor, Parijs Ilse, Pichon Bruno, Roets Ellen, Sammels Eva, Smits Guillaume, Suenaert Marion, Sznajer Yves, Van den Bogaert Kris, Vancoillie Leen, Vandeputte Lotte, Vantroys Elise, Vermeesch Joris Robert, Janssens Katrien
Аннотация
METHODS: We performed a retrospective cohort study including data from pregnant women with a twin or higher-order gestation who underwent genome-wide NIPT at one of the eight Belgian genetic centers between November 1, 2013, and March 1, 2020. Chorionicity and amnionicity were determined by ultrasonography. Follow-up invasive testing was carried out in the event of positive NIPT results. Sensitivity and specificity were calculated for the detection of trisomy 21, 18, and 13 in the dichorionic-diamniotic twin cohort.
RESULTS: Unique NIPT analyses were performed for 4,150 pregnant women with a multiple gestation and an additional 767 with vanishing gestations. The failure rate in multiple gestations excluding vanishing gestations ranged from 0% to 11.7% among the different genetic centers. Overall, the failure rate was 4.8%, which could be reduced to 1.2% after single resampling. There were no common fetal trisomies detected among the 86 monochorionic-monoamniotic and 25 triplet cases. Two monochorionic-diamniotic twins had an NIPT result indicative of a trisomy 21, which was confirmed in both fetuses. Among 2,716 dichorionic-diamniotic twin gestations, a sensitivity of 100% (95% CI 74.12-100%) and a specificity of 100% (95% CI 99.86-100%) was reached for trisomy 21 (n=12). For trisomy 18 (n=3), the respective values were 75% (95% CI 30.06-95.44%) sensitivity and 100% (95% CI 99.86-100%) specificity, and for trisomy 13 (n=2), 100% (95% CI 20.65-100%) sensitivity and 99.96% (95% CI 99.79-99.99%) specificity. In the vanishing gestation group, 28 NIPT results were positive for trisomy 21, 18, or 13, with only five confirmed trisomies.
CONCLUSION: Genome-wide NIPT performed accurately for detection of aneuploidy in dichorionic-diamniotic twin gestations.