Investigative ophthalmology & visual science · 2017
Edaravone Prevents Retinal Degeneration in Adult Mice Following Optic Nerve Injury.
СтатьяНазвание и аннотация: Перевод в очереди
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Akiyama Goichi, Azuchi Yuriko, Guo Xiaoli, Noro Takahiko, Kimura Atsuko, Harada Chikako, Namekata Kazuhiko, Harada Takayuki
Аннотация
PURPOSE: To assess the therapeutic potential of edaravone, a free radical scavenger that is used for the treatment of acute brain infarction and amyotrophic lateral sclerosis, in a mouse model of optic nerve injury (ONI).
METHODS: Two microliters of edaravone (7.2 mM) or vehicle were injected intraocularly 3 minutes after ONI. Optical coherence tomography, retrograde labeling of retinal ganglion cells (RGCs), histopathology, and immunohistochemical analyses of phosphorylated apoptosis signal-regulating kinase-1 (ASK1) and p38 mitogen-activated protein kinase (MAPK) in the retina were performed after ONI. Reactive oxygen species (ROS) levels were assessed with a CellROX Green Reagent.
RESULTS: Edaravone ameliorated ONI-induced ROS production, RGC death, and inner retinal degeneration. Also, activation of the ASK1-p38 MAPK pathway that induces RGC death following ONI was suppressed with edaravone treatment.
CONCLUSIONS: The results of this study suggest that intraocular administration of edaravone may be a useful treatment for posttraumatic complications.
METHODS: Two microliters of edaravone (7.2 mM) or vehicle were injected intraocularly 3 minutes after ONI. Optical coherence tomography, retrograde labeling of retinal ganglion cells (RGCs), histopathology, and immunohistochemical analyses of phosphorylated apoptosis signal-regulating kinase-1 (ASK1) and p38 mitogen-activated protein kinase (MAPK) in the retina were performed after ONI. Reactive oxygen species (ROS) levels were assessed with a CellROX Green Reagent.
RESULTS: Edaravone ameliorated ONI-induced ROS production, RGC death, and inner retinal degeneration. Also, activation of the ASK1-p38 MAPK pathway that induces RGC death following ONI was suppressed with edaravone treatment.
CONCLUSIONS: The results of this study suggest that intraocular administration of edaravone may be a useful treatment for posttraumatic complications.
Предметные рубрики MeSH
AnimalsAntipyrineDisease Models, AnimalEdaravoneFree Radical ScavengersImmunohistochemistryInjections, IntraocularMAP Kinase Kinase 1MAP Kinase Kinase Kinase 5MiceMice, Inbred C57BLOptic Nerve InjuriesReactive Oxygen SpeciesRetinaRetinal DegenerationRetinal Ganglion CellsTomography, Optical Coherence