Уровни экспрессии TGFB1 и IFNB1 в периферийных моноЯдерных клетках крови с множественным склерозом в отношении проксимальных rs1800469 и rs2275888 eQTLs.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zivotic Ivan, Kolic Ivana, Stefanovic Milan, Stefanovic Natasa Macak, Dincic Evica, Stankovic Aleksandra, Zivkovic Maja
Аннотация
Оригинал публикации
Association of TGFB1 and IFNB1 expression levels in peripheral blood mononuclear cells with multiple sclerosis with respect to proximal rs1800469 and rs2275888 eQTLs.
BACKGROUND: Multiple sclerosis (MS) is a central nervous system inflammatory disorder with evidence of peripheral immune system dysregulation. Complex signaling interaction of immune cytokines transforming growth factor beta 1 (TGFB1) and interferon beta 1 (IFNB1) have been reported, but not fully defined in MS. Thus, we aimed to assess association of TGFB1 and IFNB1 mRNA expression with MS onset and phase of the disease. Additionally, we investigated the genetic basis of the two under-examined genetic variants, previously designated as eQTLs for TGFB1 (rs1800469) and IFNB1 (rs2275888), with MS occurrence and expression of investigated genes.
DESIGN: Study included 417 MS patients and 293 controls for genetic association and 71 MS patients and 101 healthy controls for gene expression analysis in peripheral blood mononuclear cells (PBMCs). Analysis was performed using TaqMan® technology (Applied Biosystems, Foster City, CA, US).
RESULTS: We found significantly higher TGFB1 mRNA level in relapsing-remitting (RR) MS patients in comparison to the controls (P = 0.03). Relative IFNB1 mRNA levels in RRMS patients who were in relapse was significantly higher in comparison to those in remission (P = 0.03). A positive correlation of TGFB1 and IFNB1 mRNA levels was observed in MS patient's subgroups. Genetic variants did not affect MS occurrence or the gene expressions.
CONCLUSIONS: Current findings provide important supplementary information about the association between TGFB1 and IFNB1 gene expression and MS. Cross-talk between TGFB1 and IFNB1 might be a modulating factor of inflammatory response in different phases of RR MS. These results need to be further replicated.