Zhonghua wei zhong bing ji jiu yi xue · 2014
[The protective effect of valproic acid on myocardium in rats with lethal scald injury and its mechanism].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Luo Hongmin, Hu Sen, Bai Huiying, Wang Haibin, Du Minghua, Lin Zhilong, Ma Li, Wang Huan, Lyu Yi, Sheng Zhiyong
Аннотация
OBJECTIVE: To investigate the protective effects of valproic acid (VPA) on myocardium in rats following lethal burn injury and its mechanism.
METHODS: Seventy-eight Sprague-Dawley (SD) rats were randomly assigned to four groups: sham-scald group (n=10), sham-scald + VPA group (n=10), scald group (n=29), and scald + VPA group (n=29). Rats in the latter two groups were subjected to 55% total body surface area (TBSA) third-degree burns by immersing the back of the trunk for 15 seconds, both lower extremities for 15 seconds, and the abdomen for 8 seconds in 80 centigrade water. Sham-scald rats were immersed in 37 centigrade water instead. Rats were then subcutaneously injected with VPA (300 mg/kg) or normal saline as control. Blood of 5 rats in each group was with drawn from the abdominal aorta at 6 hours after injury for measurement of plasma creatine kinase-MB (CK-MB) activities; then the rats were sacrificed and heart tissues were harvested for the measurement of acetylated histone H3 and activated caspase-3 by Western Blot. The remaining rats were used for 12-hour survival analysis.
RESULTS: Compared with sham-scald group, there was a significant increase in plasma CK-MB activities (5 438.0 ± 413.6 U/L vs. 2 881.0 ± 324.8 U/L, P<0.05) and activated caspase-3 protein levels in heart tissue (gray value: 1.75 ± 0.25 vs. 1.00 ± 0.18, P<0.05) and an significant decline in the acetylation levels of histone H3 (gray value: 0.55 ± 0.18 vs. 1.00 ± 0.20, P<0.05) after major burn injury. VPA treatment significantly reduced the plasma CK-MB activities [(4 018.0 ± 388.3) U/L], activated caspase-3 protein levels in heart tissue (gray value: 1.33 ± 0.20), and raised the acetylation levels of histone H3 (gray value: 2.20 ± 0.23, all P<0.05). Survival analysis by Kaplan-Meier curves showed that the survival was improved after VPA treatment, and the survival rate was increased from 0 to 50% at 12 hours (P<0.05).
CONCLUSIONS: VPA can attenuate cardiac injury and improve survival in a rodent model of lethal burn injury. These protective effects may be due to its inhibitory effects on histone deacetylase and caspase-3 activation.
METHODS: Seventy-eight Sprague-Dawley (SD) rats were randomly assigned to four groups: sham-scald group (n=10), sham-scald + VPA group (n=10), scald group (n=29), and scald + VPA group (n=29). Rats in the latter two groups were subjected to 55% total body surface area (TBSA) third-degree burns by immersing the back of the trunk for 15 seconds, both lower extremities for 15 seconds, and the abdomen for 8 seconds in 80 centigrade water. Sham-scald rats were immersed in 37 centigrade water instead. Rats were then subcutaneously injected with VPA (300 mg/kg) or normal saline as control. Blood of 5 rats in each group was with drawn from the abdominal aorta at 6 hours after injury for measurement of plasma creatine kinase-MB (CK-MB) activities; then the rats were sacrificed and heart tissues were harvested for the measurement of acetylated histone H3 and activated caspase-3 by Western Blot. The remaining rats were used for 12-hour survival analysis.
RESULTS: Compared with sham-scald group, there was a significant increase in plasma CK-MB activities (5 438.0 ± 413.6 U/L vs. 2 881.0 ± 324.8 U/L, P<0.05) and activated caspase-3 protein levels in heart tissue (gray value: 1.75 ± 0.25 vs. 1.00 ± 0.18, P<0.05) and an significant decline in the acetylation levels of histone H3 (gray value: 0.55 ± 0.18 vs. 1.00 ± 0.20, P<0.05) after major burn injury. VPA treatment significantly reduced the plasma CK-MB activities [(4 018.0 ± 388.3) U/L], activated caspase-3 protein levels in heart tissue (gray value: 1.33 ± 0.20), and raised the acetylation levels of histone H3 (gray value: 2.20 ± 0.23, all P<0.05). Survival analysis by Kaplan-Meier curves showed that the survival was improved after VPA treatment, and the survival rate was increased from 0 to 50% at 12 hours (P<0.05).
CONCLUSIONS: VPA can attenuate cardiac injury and improve survival in a rodent model of lethal burn injury. These protective effects may be due to its inhibitory effects on histone deacetylase and caspase-3 activation.