Hydroxytyrosol protects sperm viability and DNA integrity in human asthenoteratozoospermia during incubation: An experimental study.

BACKGROUND

Asthenoteratozoospermia (AT), marked by poor sperm motility and abnormal morphology, is a major cause of male infertility. During semen handling, AT samples generate high reactive oxygen species (ROS) levels, increasing oxidative stress and sperm damage.

OBJECTIVE

This study investigated the effects of hydroxytyrosol (HT), a natural antioxidant, on sperm quality and ROS levels in AT samples during incubation.

MATERIALS AND

METHODS

This experimental study was conducted in 2 phases. In phase I, 20 AT samples were divided into 5 groups (0, 25, 50, 75, and 100 μg/mL HT) and incubated for 30, 45, and 60 min to determine optimal conditions based on sperm motility and viability. In phase II, another 20 AT samples were treated with 25 and 50 μg/mL HT for 30 min. Sperm mitochondrial membrane potential, intracellular ROS, DNA fragmentation, and lipid peroxidation were evaluated.

RESULTS

Sperm motility decreased after incubation; however, treatment with HT maintained motility, although the difference was not statistically significant. Similarly, sperm viability declined following incubation compared to the baseline. 25 μg/mL HT significantly preserves sperm viability after 30 min compared to 0 μg/mL HT (p = 0.02). Incubation significantly increased DNA fragmentation; however, at both 25 and 50 μg/mL HT, DNA fragmentation remained comparable to pre-incubation levels. No significant effects were observed on lipid peroxidation, ROS levels, or mitochondrial membrane potential.

CONCLUSION

Incubation of AT sperm with HT for 30 min preserved sperm viability and reduced DNA damage, suggesting that HT may serve as a beneficial antioxidant in assisted reproductive technologies for men with AT by minimizing oxidative stress-related sperm damage.