Androgen Receptor Splice Variant-7 Expression Analysis in Circulating Tumor Cells: Methodology and Application of AdnaTest in Prostate Cancer Research.

This chapter outlines a detailed methodology for the analysis of androgen receptor splice variant-7 (AR-V7) expression in circulating tumor cells (CTCs) using the AdnaTestProstate CancerSelect and ProstateCancerDetect system. The method incorporates immunomagnetic enrichment, mRNA isolation, and reverse transcription PCR to detect AR-V7, a key marker associated with treatment resistance in castration-resistant prostate cancer (CRPC). The protocol includes step-by-step instructions for sample preparation, magnetic bead-based enrichment, and gene expression analysis, providing a robust platform to investigate the molecular characteristics of CTCs and their roles in therapy resistance.