Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine · 2010
[Fetal RHD genotyping by PCR using plasma from D negative pregnant women].
Клиническое исследованиеАннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Achargui S, Tijane M, Benchemsi N
Аннотация
AIM OF THE STUDY: Free fetal DNA in maternal blood offers a non invasive method for prenatal diagnosis. The aim of this study is to perform fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
PATIENTS AND METHODS: Plasma sample from 120 D negative pregnant women from 10 to 40 gestational weeks were tested by conventional PCR for the presence of exons 7 and 10 of RHD gene. The results were compared with the RhD phenotype of the newborns.
RESULTS: In this study, the positive and the negative predictive value of the fetal RhD status were 98.7 and 96.7%, respectively.
CONCLUSION: These results demonstrate de feasibility of fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
PATIENTS AND METHODS: Plasma sample from 120 D negative pregnant women from 10 to 40 gestational weeks were tested by conventional PCR for the presence of exons 7 and 10 of RHD gene. The results were compared with the RhD phenotype of the newborns.
RESULTS: In this study, the positive and the negative predictive value of the fetal RhD status were 98.7 and 96.7%, respectively.
CONCLUSION: These results demonstrate de feasibility of fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.