[Fetal RHD genotyping by PCR using plasma from D negative pregnant women].

AIM OF THE STUDY: Free fetal DNA in maternal blood offers a non invasive method for prenatal diagnosis. The aim of this study is to perform fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.

PATIENTS AND

METHODS

Plasma sample from 120 D negative pregnant women from 10 to 40 gestational weeks were tested by conventional PCR for the presence of exons 7 and 10 of RHD gene. The results were compared with the RhD phenotype of the newborns.

RESULTS

In this study, the positive and the negative predictive value of the fetal RhD status were 98.7 and 96.7%, respectively.

CONCLUSION

These results demonstrate de feasibility of fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.