Bioinformatics (Oxford, England) · 2025
AntigenApp: a laboratory data management system for nanobody generation and sequence analysis.
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Lubbock Alexander L R, Eyssen Lauren E-A, Parker Kelly, Basham Mark, Shemilt Laura A, Owens Raymond J
Аннотация
MOTIVATION: Nanobodies are single domain antibodies derived from the unique heavy chain-only immunoglobulins of the camelid family, and have broad applications in biosciences, including as imaging and diagnostic agents. The generation of nanobodies is a multi-stage experimental process for which we have developed AntigenApp a laboratory data management system for nanobody generation and sequence analysis.
RESULTS: AntigenApp is a web application and database built to capture data generated during all steps of the nanobody discovery pipeline and to provide built-in analysis capabilities of the experimental results. This includes enzyme-linked immunosorbent assay optical density measurements, sequencing results, nanobody domain labelling, and search/clustering capabilities using protein BLAST. The tool incorporates a standardized naming convention that is automatically applied to nanobodies stored in the database and we envisage the tool being of interest to the wider nanobody community for in-house use.
AVAILABILITY AND IMPLEMENTATION: AntigenApp is available from https://github.com/rosalindfranklininstitute/antigen-app under the Apache 2.0 licence and published https://doi.org/10.5281/zenodo.17397055.
RESULTS: AntigenApp is a web application and database built to capture data generated during all steps of the nanobody discovery pipeline and to provide built-in analysis capabilities of the experimental results. This includes enzyme-linked immunosorbent assay optical density measurements, sequencing results, nanobody domain labelling, and search/clustering capabilities using protein BLAST. The tool incorporates a standardized naming convention that is automatically applied to nanobodies stored in the database and we envisage the tool being of interest to the wider nanobody community for in-house use.
AVAILABILITY AND IMPLEMENTATION: AntigenApp is available from https://github.com/rosalindfranklininstitute/antigen-app under the Apache 2.0 licence and published https://doi.org/10.5281/zenodo.17397055.