Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals · 2009
The acute-phase protein serum amyloid A3 is expressed in the bovine mammary gland and plays a role in host defence.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Molenaar Adrian J, Harris D Paul, Rajan Gillian H, Pearson Monica L, Callaghan Megan R, Sommer Lilly, Farr Vicki C, Oden Kim E, Miles Michelle C, Petrova Rosica S, Good Laura L, Singh Kuljeet, McLaren Robert D, Prosser Colin G, Kim Kwang S, Wieliczko Robert J, Dines Mark H, Johannessen Kirsti M, Grigor Murray R, Davis Steven R, Stelwagen Kerst
Аннотация
The serum amyloid A protein is one of the major reactants in the acute-phase response. Using representational difference analysis comparing RNA from normal and involuting quarters of a dairy cow mammary gland, we found an mRNA encoding the SAA3 protein (M-SAA3). The M-SAA3 mRNA was localized to restricted populations of bovine mammary epithelial cells (MECs). It was expressed at a moderate level in late pregnancy, at a low level through lactation, was induced early in milk stasis, and expressed at high levels in most MECs during mid to late involution and inflammation/mastitis. The mature M-SAA3 peptide was expressed in Escherichia coli, antibodies made, and shown to have antibacterial activity against E. coli, Streptococcus uberis and Pseudomonas aeruginosa. These results suggest that the mammary SAA3 may have a role in protection of the mammary gland during remodelling and infection and possibly in the neonate gastrointestinal tract.