Ultrastructure assessment of oocyte from mouse with polycystic ovary syndrome following cryopreservation.

BACKGROUND

The polycystic ovary syndrome (PCOS) is a substantial obstacle to female fertility due to ovulation inhibition. Oocyte cryopreservation is crucial for preserving fertility in women with fertility-compromising disorders such as PCOS.

OBJECTIVE

In this study, the ultrastructural damages of oocytes were evaluated following freezing from PCOS mouse model.

MATERIALS AND

METHODS

This experimental study was conducted on 30 adult NMRI mouse. The study comprised three groups: 1) unfrozen PCOS oocytes; 2) vitrified-thawed control oocytes; and 3) vitrified-thawed PCOS oocytes. Transmission electron microscopy was employed for ultrastructure examination across all groups. Moreover, the expression of apoptotic genes, including BAX and Bcl2, was assessed using real time-quantitative polymerase chain reaction (RT-PCR).

RESULTS

The oocyte cryopreservation process had a high impact on the destruction of ooplasm cortical granules, Golgi complexes and mitochondria in vitrified-thawed PCOS oocytes compared to the other groups. In PCOS oocytes, particularly those that were vitrified-thawed, there was a notable increase in vacuolation, with a higher abundance of larger and more numerous vacuoles observed compared to the control group. The vitrified-thawed PCOS group also exhibited a notable increase in the expression of the apoptotic gene compared to the other groups (p < 0.05).

CONCLUSION

A precise evaluation of oocyte cryopreservation is imperative for improving this technique and for producing high-quality oocytes with enhanced fertility potential. This study contributes valuable insights into understanding the intricate relationship between PCOS, cryopreservation and oocyte quality. https://doi.org/10.54680/fr25210110412.