The Journal of hospital infection · 2026
Evaluation of 15-second alcohol-based hand rub efficacy: a multi-laboratory study using a modified EN 1500 protocol.
СтатьяМногоцентровое исследованиеРандомизированное исследованиеНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Roesch K-M, Gebel J, Bolten A, Cavalleri M, Christiansen B, Droop F, Eilts B, Exner M, Gabriel H, Hildebrandt C, Koburger-Janssen T, Konrat K, Lee C S, Lenz J, Martiny H, Meckel M, Mutters N T, Pahl S, Paßvogel L, Schartner C, Seyringer F, Steinhauer K, Vecchio L J, Vossebein L, Wille A, Kramer A, Suchomel M
Аннотация
INTRODUCTION: Hands are a key vector for pathogen transmission in healthcare, making effective hand antisepsis crucial for infection prevention. According to the European standard EN 1500, the reference method for evaluating hand antiseptics, a minimum rub-in time of 30 s is required. However, observations show healthcare workers typically spend less time on hand antisepsis.
METHOD: To assess the feasibility of a reduced rub-in time under standardized conditions, the German Association for Applied Hygiene conducted a multi-centre ring trial in 14 laboratories using a modified EN 1500 protocol (15 s, 3 mL of 60% v/v propan-2-ol). In a randomized crossover design, volunteers' hands were contaminated with Escherichia coli K12 and treated either with the reference (2 × 3 mL/2 × 30 s) or the test protocol (1 x 3 mL/15 s). Microbial reduction was measured and non-inferiority statistically analysed.
RESULTS: The 15-second protocol yielded significantly lower log10 reductions than the reference in 13 out of 14 laboratories but demonstrated consistent reproducibility and satisfactory interlaboratory performance. Challenges in completing the full rub-in technique within 15 s were reported, indicating the need for targeted training.
CONCLUSION: These findings support the methodological feasibility of a shortened protocol and are consistent with evolving clinical guidelines advocating reduced rub-in times, as well as with real-world practice, where healthcare workers typically spend less than 30 s on hand antisepsis. Nonetheless, any revision of EN 1500 should proceed cautiously to ensure antimicrobial efficacy, emphasizing complete hand coverage and strict adherence to technique.
METHOD: To assess the feasibility of a reduced rub-in time under standardized conditions, the German Association for Applied Hygiene conducted a multi-centre ring trial in 14 laboratories using a modified EN 1500 protocol (15 s, 3 mL of 60% v/v propan-2-ol). In a randomized crossover design, volunteers' hands were contaminated with Escherichia coli K12 and treated either with the reference (2 × 3 mL/2 × 30 s) or the test protocol (1 x 3 mL/15 s). Microbial reduction was measured and non-inferiority statistically analysed.
RESULTS: The 15-second protocol yielded significantly lower log10 reductions than the reference in 13 out of 14 laboratories but demonstrated consistent reproducibility and satisfactory interlaboratory performance. Challenges in completing the full rub-in technique within 15 s were reported, indicating the need for targeted training.
CONCLUSION: These findings support the methodological feasibility of a shortened protocol and are consistent with evolving clinical guidelines advocating reduced rub-in times, as well as with real-world practice, where healthcare workers typically spend less than 30 s on hand antisepsis. Nonetheless, any revision of EN 1500 should proceed cautiously to ensure antimicrobial efficacy, emphasizing complete hand coverage and strict adherence to technique.