Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases · 1995
[Formation of experimental rat bladder calculus and adherence of Pseudomonas aeruginosa to the calculus].
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Takahata M, Kurose S, Shinmura Y, Watanabe Y, Narita H, Hasegawa M
Аннотация
The formation of experimental bladder calculus was studied. The calculus was formed by the uptake of ethylene glycolwater (1%) and retaining the silk thread in rat bladder with high frequency. The components of the calculus were calcium oxalate and calcium phosphate from the results of the electron prove micro analysis (EPMA) and ion chromatography. On the 7th day after the beginning of experiment, Pseudomonas aeruginosa was inoculated to the rat bladder via the urethra. Seven days after the infection, P. aeruginosa adhered to the surface of the calculus such as an aspect of a biofilm. It was considered that this experimental model was useful to study the adherence of bacteria, biofilm formation and its chemotherapy by antibacterial agents.