Analytica chimica acta · 2026
A ratiometric fluorescent probe consisting of Zn-MOF and calcium ion for sensitive and visual detection of doxycycline.
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Yang Nan, Kang Le, Yao Wei, Xu Baotong, Feng Yunhui, Fedin Vladimir P, Gao Enjun
Аннотация
BACKGROUND: The global consumption of antibiotics is steadily rising, with doxycycline being extensively utilized in both animal husbandry and the treatment of COVID-19. Overuse of this antibiotic may result in residual accumulation and pose potential health risks. Conventional detection methods are often complex and labor-intensive, whereas fluorescence analysis offers advantages such as high sensitivity and rapid response. In this work, the development of a simple and efficient MOF-based fluorescent sensor for the detection of doxycycline holds considerable promise for practical applications.
RESULTS: In this paper, a unique 3D metal-organic framework Zn[C18H16O4N4]·C2H6SO·2H2O (Zn-MOF) for rapid detection of doxycycline was successfully synthesized and analyzed by SEM, PXRD, FTIR, TGA and UV-vis. The fluorescence of Zn-MOF at 430 nm is quenched by the static quenching and inner filter effect between Zn-MOF and doxycycline with detection limit of 2.01 μM. Concurrently, Ca2+ can combine with doxycycline and form the Zn-MOF that generates a new fluorescence signal at 550 nm. By mixing the Zn-MOF and Ca2+, a ratiometric fluorescent probe can be created. The ratio of fluorescence intensity (F550/F430) to doxycycline concentration revealed a good linear relationship and detection limit of 1.25 μM. The fluorescence detection efficiency of the ratiometric fluorescent probe is almost twice that of the single metal-organic framework.
SIGNIFICANCE: The ratiometric fluorescent probe also exhibited excellent selectivity and stability in both ionic and antibiotic solution. As with the concentration of doxycycline increase, the color of the probe and Ca2+ shifted from blue to yellow, allowing for visual detection of doxycycline and facilitating its application in rapid detection in real life.
RESULTS: In this paper, a unique 3D metal-organic framework Zn[C18H16O4N4]·C2H6SO·2H2O (Zn-MOF) for rapid detection of doxycycline was successfully synthesized and analyzed by SEM, PXRD, FTIR, TGA and UV-vis. The fluorescence of Zn-MOF at 430 nm is quenched by the static quenching and inner filter effect between Zn-MOF and doxycycline with detection limit of 2.01 μM. Concurrently, Ca2+ can combine with doxycycline and form the Zn-MOF that generates a new fluorescence signal at 550 nm. By mixing the Zn-MOF and Ca2+, a ratiometric fluorescent probe can be created. The ratio of fluorescence intensity (F550/F430) to doxycycline concentration revealed a good linear relationship and detection limit of 1.25 μM. The fluorescence detection efficiency of the ratiometric fluorescent probe is almost twice that of the single metal-organic framework.
SIGNIFICANCE: The ratiometric fluorescent probe also exhibited excellent selectivity and stability in both ionic and antibiotic solution. As with the concentration of doxycycline increase, the color of the probe and Ca2+ shifted from blue to yellow, allowing for visual detection of doxycycline and facilitating its application in rapid detection in real life.