Pakistan journal of pharmaceutical sciences · 2026
A new method for evaluating drug efficacy at the gene expression level.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Chen Mosi, Chen Genlin, Li Qi, Dai Li, Tian Libing, Shao Shuai, Yu Dehong, Su Chenglan
Аннотация
BACKGROUND: Many natural medicines exert therapeutic effects through multitarget regulation, and using methods that simply evaluate the effects of a chemical drug on several targets to study natural products may not comprehensively reflect their efficacy.
OBJECTIVES: This research attempts to explore and establish a new method to evaluate drug efficacy at the gene expression level using natural hepatoprotective drugs.
METHODS: Forty SPF male SD rats were randomly divided into a control group, a liver injury model group, a positive drug group (reduced glutathione), a curcumin treatment group and a puerarin treatment group. Carbon tetrachloride was used to damage liver cells and establish a liver injury model. Gene microarray technology was used to detect gene expression in liver cells.
RESULTS: Drug treatments significantly reduced the number of differentially expressed genes in the liver compared with model group and efficacy evaluation of drugs were curcumin > positive control drug > puerarin. The liver function indices and pathological analysis also supported the above results.
CONCLUSION: Our findings demonstrated that the efficacy of different drugs can be evaluated at the level of gene expression.
OBJECTIVES: This research attempts to explore and establish a new method to evaluate drug efficacy at the gene expression level using natural hepatoprotective drugs.
METHODS: Forty SPF male SD rats were randomly divided into a control group, a liver injury model group, a positive drug group (reduced glutathione), a curcumin treatment group and a puerarin treatment group. Carbon tetrachloride was used to damage liver cells and establish a liver injury model. Gene microarray technology was used to detect gene expression in liver cells.
RESULTS: Drug treatments significantly reduced the number of differentially expressed genes in the liver compared with model group and efficacy evaluation of drugs were curcumin > positive control drug > puerarin. The liver function indices and pathological analysis also supported the above results.
CONCLUSION: Our findings demonstrated that the efficacy of different drugs can be evaluated at the level of gene expression.